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JoVE Journal Neuroscience
En roman In Vitro Live-imaging analyse af Astrocyte-medieret fagocytose bruger pH indikator-konjugeret Synaptosomes
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En roman In Vitro Live-imaging analyse af Astrocyte-medieret fagocytose bruger pH indikator-konjugeret Synaptosomes

Article DOI: 10.3791/56647-v • 06:43 min • February 5th, 2018
February 5th, 2018 •
1, 1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

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Denne protokol udgør en in vitro- live imaging fagocytose assay for at måle astrocytter fagocyterende kapacitet. Renset rotte astrocytter og mikroglia bruges sammen med pH indikator-konjugeret synaptosomes. Denne metode kan registrere real-time engulfment og nedbrydning kinetik og giver en passende screening platform til at identificere faktorer modulere astrocyte fagocytose.

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Neurovidenskab sag 132 Astrocyte mikroglia synaptosome pH indikator fagocytose engulfment nedbrydning live imaging
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