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En roman In Vitro Live-imaging analyse af Astrocyte-medieret fagocytose bruger pH indikator-konjugeret Synaptosomes
 

En roman In Vitro Live-imaging analyse af Astrocyte-medieret fagocytose bruger pH indikator-konjugeret Synaptosomes

Article doi: 10.3791/56647
February 5th, 2018 Usage Statistics

Summary February 5th, 2018

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Denne protokol udgør en in vitro- live imaging fagocytose assay for at måle astrocytter fagocyterende kapacitet. Renset rotte astrocytter og mikroglia bruges sammen med pH indikator-konjugeret synaptosomes. Denne metode kan registrere real-time engulfment og nedbrydning kinetik og giver en passende screening platform til at identificere faktorer modulere astrocyte fagocytose.

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