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JoVE Journal Neuroscience
Ein neuartiges In vitro- Live-Imaging Assay Astrozyten vermittelt Phagozytose mittels pH Indikator konjugiert Synaptosomes
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Ein neuartiges In vitro- Live-Imaging Assay Astrozyten vermittelt Phagozytose mittels pH Indikator konjugiert Synaptosomes

Article DOI: 10.3791/56647-v • 06:43 min • February 5th, 2018
February 5th, 2018 •
1, 1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

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Dieses Protokoll stellt einen in-vitro- live-Imaging Phagozytose Assay phagocytic Kapazität von Astrozyten zu messen. Gereinigte Ratte Astrozyten und Mikroglia sind zusammen mit pH-Indikator-konjugiert Synaptosomes verwendet. Diese Methode kann in Echtzeit Engulfment und Abbau Kinetik erkennen und bietet eine geeignete Screening-Plattform für Astrozyten Phagozytose modulierende Faktoren zu identifizieren.

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Neurowissenschaften Ausgabe 132 Astrozyten Mikroglia Synaptosome pH-Indikator Phagozytose Verwicklung Abbau live-imaging
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