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En roman In Vitro Live-imaging test av Astrocyten-medierad fagocytos med pH-indikator-konjugerad Synaptosomes

Article DOI: 10.3791/56647-v • 06:43 min • February 5th, 2018
February 5th, 2018 •
1, 1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

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Detta protokoll presenterar ett in vitro- live-imaging fagocytos test för att mäta fagocytos kapacitet av astrocyter. Renat råtta astrocyter och mikroglia används tillsammans med pH-indikator-konjugerad synaptosomes. Denna metod kan upptäcka realtid omvälvning och nedbrytning kinetik och ger en lämplig screening plattform för att identifiera faktorer som modulerande Astrocyten fagocytos.

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Neurovetenskap fråga 132 astrocyt mikroglia synaptosome pH-indikator fagocytos omvälvning nedbrytning live-imaging
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