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Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy
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Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
Journal (Biology)
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Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy

Article DOI: 10.3791/56666-v • 10:03 min • November 30th, 2017
November 30th, 2017 •
1, 1,2,3, 2, 4, 2, 4, 3, 1
1Department of Radiation Biology, Institute of Aerospace Medicine, Space Microbiology Research Group, German Aerospace Center (DLR e.V.), 2Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Ruhr-University Bochum, 3Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Biomedical Applications of Plasma Technology, Ruhr-University Bochum, 4Advanced Light and Electron Microscopy (ZBS 4), Robert Koch Institute

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Summary
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This protocol illustrates the important consecutive steps required to assess the relevance of monitoring vitality parameter and DNA repair processes in reviving Bacillus subtilis spores after treatment with low pressure plasma by tracking fluorescence-labelled DNA repair proteins via time-resolved confocal microscopy and scanning electron microscopy.

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Low Pressure Plasma Sterilization Bacillus Subtilis Spores Live Cell Microscopy DNA Repair Confocal Fluorescence Microscopy Scanning Electron Microscopy Microbial Inactivation Sterilization Detrimental Effect Spore Production Shafer Sporulation Medium Centrifugation Purity Germination Status
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