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JoVE Journal
Biology

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用活细胞显微镜研究低压等离子体灭菌对枯草芽孢杆菌孢子存活的不利影响
 
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用活细胞显微镜研究低压等离子体灭菌对枯草芽孢杆菌孢子存活的不利影响

Article DOI: 10.3791/56666-v 10:03 min November 30th, 2017
November 30th, 2017

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Summary

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Click here for the English version.

这项协议说明了评估在用低压等离子体处理后恢复枯草芽孢杆菌孢子后, 监测活力参数和 DNA 修复过程的相关性所需的重要连续步骤。荧光标记的 DNA 修复蛋白质通过时间分辨共焦显微镜和扫描电子显微镜。

Tags

细胞生物学 问题 129 等离子灭菌 去污,枯草芽孢杆菌 孢子抵抗 DNA 修复 活细胞成像 SEM cLSM 荧光显微镜
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