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Undersøke den skadelige effekter av lav press plasmasterilisering på overlevelse av Bacillus subtilis Spores ved hjelp av Live celle mikroskopi
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Undersøke den skadelige effekter av lav press plasmasterilisering på overlevelse av Bacillus subtilis Spores ved hjelp av Live celle mikroskopi

Article DOI: 10.3791/56666-v • 10:03 min • November 30th, 2017
November 30th, 2017 •
1, 1,2,3, 2, 4, 2, 4, 3, 1
1Department of Radiation Biology, Institute of Aerospace Medicine, Space Microbiology Research Group, German Aerospace Center (DLR e.V.), 2Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Ruhr-University Bochum, 3Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Biomedical Applications of Plasma Technology, Ruhr-University Bochum, 4Advanced Light and Electron Microscopy (ZBS 4), Robert Koch Institute

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Denne protokollen illustrerer viktige sammenhengende trinn kreves for å vurdere relevansen av vitalitet parameteren og DNA reparasjon prosesser i gjenopplive Bacillus subtilis spores etter behandling med lavtrykk plasma av sporing fluorescens-merket DNA reparasjon proteiner via gang-løst AC confocal mikroskopi og skanning elektronmikroskop.

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Mobilnettet biologi problemet 129 plasmasterilisering dekontaminering Bacillus subtilis spore motstand DNA-reparasjon levende celle imaging SEM cLSM fluorescens mikroskopi
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