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Journal / Neuroscience / 血液中 n-甲基-d-天门冬氨酸 (NMDA)…
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Neuroscience

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JoVE Journal Neuroscience
血液中 n-甲基-d-天门冬氨酸 (NMDA) 受体自身抗体的简易细胞免疫荧光检测
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血液中 n-甲基-d-天门冬氨酸 (NMDA) 受体自身抗体的简易细胞免疫荧光检测

Article DOI: 10.3791/56676-v • 07:20 min • January 9th, 2018
January 9th, 2018 •
1,2, 1
1Department of Psychiatry, Chang Gung Memorial Hospital-Linkou, 2Department and Graduate Institute of Biomedical Sciences, Chang Gung University

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我们 ectopically 表达了以绿色荧光蛋白标记的 nmda 受体在人胚细胞 (HEK293) 中的 NR1 亚基作为抗原, 以检测疑似自身免疫性脑炎患者血液中 nmda 受体的自身抗体。这种简单的方法可能适用于临床设置的筛选目的。

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神经科学 问题 131 抗 NMDA 受体自身抗体 NR1 亚基 绿色荧光蛋白 自身免疫性脑炎 细胞免疫荧光法 鉴别诊断 精神症状
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