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Neuroscience

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JoVE Journal Neuroscience
En enkel Cell-baserad immunofluorescens analysen att upptäcka autoantikroppar mot N-metyl-D-aspartat (NMDA)-receptorn i blod
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En enkel Cell-baserad immunofluorescens analysen att upptäcka autoantikroppar mot N-metyl-D-aspartat (NMDA)-receptorn i blod

Article DOI: 10.3791/56676-v • 07:20 min • January 9th, 2018
January 9th, 2018 •
1,2, 1
1Department of Psychiatry, Chang Gung Memorial Hospital-Linkou, 2Department and Graduate Institute of Biomedical Sciences, Chang Gung University

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Vi uttryckte ectopically NR1-subenheten av NMDA-receptorn taggade med grönt fluorescerande protein i mänskliga embryonala celler (HEK293) som antigen att upptäcka autoantikroppar mot NMDA-receptorn i blodet hos patienter med misstänkt med autoimmun encefalit. Denna enkla metod kan vara lämplig vid screening i kliniska inställningar.

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Neurovetenskap fråga 131 Anti-NMDA-receptorn autoantikroppar NR1-subenheten grönt fluorescerande protein autoimmun encefalit cellbaserade immunofluorescens assay differentiell diagnos neuropsykiatriska symtom
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