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Journal / Immunology and Infection / Bygga upp en High-throughput Screening plattform…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Bygga upp en High-throughput Screening plattform att bedöma heterogenitet HER2 genen amplifiering i bröstcancer
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Bygga upp en High-throughput Screening plattform att bedöma heterogenitet HER2 genen amplifiering i bröstcancer

Article DOI: 10.3791/56686-v • 11:34 min • December 5th, 2017
December 5th, 2017 •
1, 1,2, 1,3, 1,2, 4, 5, 1, 4, 1, 1,6
1Division of Pathology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 2School of Medicine, University of Milan, 3School of Biology, University of Pavia, 4Division of Breast Surgery, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, 5Division of Medical Oncology, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, 6Department of Biomedical, Surgical, and Dental Sciences, University of Milan

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Heterogen fördelning av HER2-positiva celler kan observeras i en delmängd av bröstcancer och genererar kliniska dilemman. Här introducerar vi en pålitlig och kostnadseffektiv protokoll för att definiera, kvantifiera och jämföra HER2 intra-tumör genetisk heterogenitet i en stor serie av heterogeneously bearbetade bröstcancer.

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Immunologi fråga 130 bröstcancer human epidermal tillväxtfaktor receptor 2 (HER2) heterogenitet vävnad microarray i situ hybridisering hög genomströmning molekylär analys
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