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Journal / Biochemistry / Rilevamento di fosforilazione di Rab10 di LRRK2…
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Biochemistry

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JoVE Journal Biochemistry
Rilevamento di fosforilazione di Rab10 di LRRK2 attività mediante SDS-PAGE con un Tag di fosfato-binding
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Rilevamento di fosforilazione di Rab10 di LRRK2 attività mediante SDS-PAGE con un Tag di fosfato-binding

Article DOI: 10.3791/56688-v • 08:55 min • December 14th, 2017
December 14th, 2017 •
1, 1,2
1Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, The University of Tokyo

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Lo studio presente descrive un metodo semplice per rilevare i livelli endogeni di Rab10 fosforilazione dalla leucina-ricche ripetere chinasi 2.

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Biochimica problema 130 morbo di Parkinson ricchi di leucina ripetere chinasi 2 Rab10 fosforilazione Phos-tag SDS-PAGE immunoblotting
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