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Immunology and Infection

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JoVE Journal Immunology and Infection
Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation
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Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation

Article DOI: 10.3791/56689-v • 10:35 min • December 26th, 2017
December 26th, 2017 •
1, 1
1British Columbia Children's Hospital Research Institute, University of British Columbia

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Antibody-based drugs have revolutionized treatment for inflammatory diseases. In addition to having direct effects on specific targets, antibodies can activate macrophages to become anti-inflammatory. This protocol describes how anti-inflammatory macrophage activation can be assessed in vitro, using mouse bone marrow macrophages, and in vivo, using peritoneal macrophages.

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Antibody-based Drugs Macrophage Activation Immunology IL-10 Producing Anti-inflammatory Activation State Mouse Bone Marrow Peritoneal Macrophages In Vitro In Vivo Sterile Hood Euthanized Mouse Foam Board 70% Ethanol Shallow Cut Skin And Fur Removal Tibias And Femurs Bone And Muscle Dissection Bone Flesh Medium Six Well Plate 26-gauge Needle
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