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Journal / Genetics / 昆虫細胞 (sf9)…
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Genetics

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JoVE Journal Genetics
昆虫細胞 (sf9) 蛋白質機能の試金のための外国遺伝子の一過性発現
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昆虫細胞 (sf9) 蛋白質機能の試金のための外国遺伝子の一過性発現

Article DOI: 10.3791/56693-v • 11:12 min • February 22nd, 2018
February 22nd, 2018 •
1, 2, 2, 3, 1
1Department of Biotechnology and Animal Science, National Ilan University, 2Department of Agricultural Biology, College of Agriculture Life Sciences, Chonbuk National University, 3Department of Entomology, National Taiwan University

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このプロトコルを記述する外国蛋白質を表現するまたは潜在的な外国蛋白質とその切り捨てられたのアミノ酸の抗アポトーシス活性を評価に使用することができます熱衝撃によるタンパク質発現システム (pDHsp/V5-彼/sf9 細胞システム)昆虫細胞での酸。

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遺伝学、問題 132、一過性発現、蛋白質機能の試金、sf9 細胞株における生殖巣 xylina複数核、アポトーシス - 3 Ly inhinbitorショウジョウバエ熱ショック 70 プロモーター、pDHsp/V5、彼のショウジョウバエ死神、アクチノマイシン D
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