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Met behulp van CRISPR/Cas9 Gene bewerken om te onderzoeken de oncogene activiteit van Mutant Calreticulin in Cytokine afhankelijke hematopoietische cellen
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Met behulp van CRISPR/Cas9 Gene bewerken om te onderzoeken de oncogene activiteit van Mutant Calreticulin in Cytokine afhankelijke hematopoietische cellen

Article DOI: 10.3791/56726-v • 10:21 min • January 5th, 2018
January 5th, 2018 •
1, 1,2,3
1Division of Hematology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 2Broad Institute, 3Dana-Farber Cancer Institute, Harvard Medical School

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Gerichte gene bewerken met behulp van CRISPR/Cas9, heeft het inzicht in de biologische functies van genen aanzienlijk vergemakkelijkt. Hier, gebruiken we de methode van de CRISPR/Cas9 voor model calreticulin mutaties in cytokine-afhankelijke hematopoietische cellen om hun oncogene activiteit te bestuderen.

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Kankeronderzoek kwestie 131 CRISPR Cas9 gene bewerken sgRNA calreticulin sequencing verslaggever
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