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JoVE Journal Cancer Research
Mit CRISPR/Cas9 gen untersuchen die Onkogene Aktivität des mutierten Calreticulin in Cytokine abhängigen hämatopoetischen Zellen bearbeiten
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Mit CRISPR/Cas9 gen untersuchen die Onkogene Aktivität des mutierten Calreticulin in Cytokine abhängigen hämatopoetischen Zellen bearbeiten

Article DOI: 10.3791/56726-v • 10:21 min • January 5th, 2018
January 5th, 2018 •
1, 1,2,3
1Division of Hematology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 2Broad Institute, 3Dana-Farber Cancer Institute, Harvard Medical School

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Gezielte gen bearbeiten CRISPR/Cas9 hat das Verständnis der biologischen Funktionen der Gene erheblich erleichtert. Hier nutzen wir die CRISPR/Cas9-Methodik zu Modell Calreticulin Mutationen in hämatopoetischen Zellen Zytokin-abhängige um ihre onkogenen Aktivität zu studieren.

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Cancer Research Ausgabe 131 CRISPR Cas9 gen bearbeiten SgRNA Calreticulin Sequenzierung reporter
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