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JoVE Journal Cancer Research
Electroforesis en Gel bidimensional juntada con métodos de espectrometría de masas para el análisis del proteoma de tejido humano Adenoma pituitario
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Electroforesis en Gel bidimensional juntada con métodos de espectrometría de masas para el análisis del proteoma de tejido humano Adenoma pituitario

Article DOI: 10.3791/56739-v • 12:34 min • April 2nd, 2018
April 2nd, 2018 •
1,2,3,4, 1,2,3, 1,2,3
1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, 4The State Key Laboratory of Medical Genetics, Central South University

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Aquí, presentamos una electroforesis en gel bidimensional (2DE) acoplada con espectrometría de masas (MS) para separar e identificar adenoma pituitario humano tejido proteoma, que presenta un patrón de 2DE buena y reproducible. Muchas proteínas se observan en cada punto de 2DE al analizar el proteoma del complejo del cáncer con el uso de MS de alta sensibilidad.

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Enfoque de Cancer Research número 134 electroforesis en Gel bidimensional isoeléctrico electroforesis en Gel de acrilamida plata tinción de Coomassie análisis de imagen huella digital masa del péptido (PMF) Spectrometry total en tándem (MS/MS) pituitaria Adenoma proteoma
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