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Journal / Cancer Research / Todimensjonal Gel geleelektroforese kombinert med …
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Todimensjonal Gel geleelektroforese kombinert med massespektrometri metoder for en analyse av menneskelige hypofysen adenom vev proteom
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Todimensjonal Gel geleelektroforese kombinert med massespektrometri metoder for en analyse av menneskelige hypofysen adenom vev proteom

Article DOI: 10.3791/56739-v • 12:34 min • April 2nd, 2018
April 2nd, 2018 •
1,2,3,4, 1,2,3, 1,2,3
1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, 4The State Key Laboratory of Medical Genetics, Central South University

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Her presenterer vi en todimensjonal gel geleelektroforese (2DE) kombinert med massespektrometri (MS) til å skille og identifisere menneskelige hypofysen adenom vev proteom, som presenterer en god og reproduserbar 2DE mønster. Mange proteiner er observert i hver 2DE spot når analysere komplekse kreft proteom med bruk av høy følsomhet MS.

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Kreftforskning problemet 134 todimensjonal Gel geleelektroforese Isoelectric fokusere akrylamid Gel geleelektroforese sølv flekker Coomassie flekker Image Analysis peptid masse fingeravtrykk (PMF) Tandem massespektrometri (MS-/ MS) hypofysen Adenoma proteom
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