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Todimensjonal Gel geleelektroforese kombinert med massespektrometri metoder for en analyse av menneskelige hypofysen adenom vev proteom
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April 02, 2018
DOI:
1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital,Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital,Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital,Central South University, 4The State Key Laboratory of Medical Genetics,Central South University
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Her presenterer vi en todimensjonal gel geleelektroforese (2DE) kombinert med massespektrometri (MS) til å skille og identifisere menneskelige hypofysen adenom vev proteom, som presenterer en god og reproduserbar 2DE mønster. Mange proteiner er observert i hver 2DE spot når analysere komplekse kreft proteom med bruk av høy følsomhet MS.
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Zhan, X., Huang, Y., Long, Y. Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome. J. Vis. Exp. (134), e56739, doi:10.3791/56739 (2018).
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