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Tvådimensionell gelelektrofores tillsammans med masspektrometri metoder för en analys av mänskliga hypofysen adenom vävnad proteomet
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Tvådimensionell gelelektrofores tillsammans med masspektrometri metoder för en analys av mänskliga hypofysen adenom vävnad proteomet

Article DOI: 10.3791/56739-v • 12:34 min • April 2nd, 2018
April 2nd, 2018 •
1,2,3,4, 1,2,3, 1,2,3
1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, 4The State Key Laboratory of Medical Genetics, Central South University

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Här presenterar vi en tvådimensionell gelelektrofores (2DE) i kombination med masspektrometri (MS) för att separera och identifiera mänskliga hypofysen adenom vävnad proteomet, som presenterar ett bra och reproducerbara 2DE mönster. Många proteiner kan observeras i varje 2DE plats när analysera komplexa cancer proteomet med användning av hög känslighet MS.

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Cancerforskning fråga 134 tvådimensionell gelelektrofores isoelektrisk fokusering akrylamid gelelektrofores silverfärgning Coomassie färgning bildanalys peptid massa fingeravtryck (PMF) Tandem Mass Spectrometry (MS/MS) hypofys Adenom proteomet
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