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Journal / Cancer Research / İnsan hipofiz adenom doku Proteom analizi için…
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JoVE Journal Cancer Research
İnsan hipofiz adenom doku Proteom analizi için kütle spektrometresi yöntemleri ile birleştiğinde iki boyutlu Jel Elektroforez
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İnsan hipofiz adenom doku Proteom analizi için kütle spektrometresi yöntemleri ile birleştiğinde iki boyutlu Jel Elektroforez

Article DOI: 10.3791/56739-v • 12:34 min • April 2nd, 2018
April 2nd, 2018 •
1,2,3,4, 1,2,3, 1,2,3
1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, 4The State Key Laboratory of Medical Genetics, Central South University

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Burada, kütle spektrometresi (ayırmak ve bir iyi ve tekrarlanabilir 2DE desen sunar insan hipofiz adenom doku Proteom tanımlamak için MS) ile birleştiğinde bir iki boyutlu Jel Elektroforez (2DE) mevcut. Çoğu protein her 2DE yerde karmaşık kanser Proteom yüksek-duyarlı MS kullanımı ile analiz ederken gözlenir.

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Kanser araştırmaları sayı: 134 iki boyutlu Jel Elektroforez Isoelectric odaklanarak Akrilamid Jel Elektroforez gümüş boyama Coomassie boyama görüntü analizi peptid kitle parmak izi (PMF) Tandem kütle spektrometresi (MS/MS) hipofiz Adenom Proteom
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