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En effektiv In Vitro gennemførelse metode af en Transcriptionally regulerede sovende skønhed System pakket ind i en Integration defekte Lentiviral vektor
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En effektiv In Vitro gennemførelse metode af en Transcriptionally regulerede sovende skønhed System pakket ind i en Integration defekte Lentiviral vektor

Article DOI: 10.3791/56742-v • 10:31 min • January 12th, 2018
January 12th, 2018 •
1, 1, 1
1Centre for Regenerative Medicine, Department of Life Sciences, University of Modena and Reggio Emilia

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Denne protokol beskriver en metode til at opnå stabile integration af et gen af interesse i det menneskelige genom transcriptionally regulerede Tornerose systemet. Forberedelse af integrationen af defekte lentiviral vektorer, in vitro- transduktion af menneskelige celler og molekylær analysen på transduced celler er rapporteret.

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Molekylærbiologi sag 131 Tornerose transposon hyperaktive transposase transkriptionel regulering Tet-ON system integration defekte lentiviral vektor in vitro- gennemførelse
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