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JoVE Journal Developmental Biology
Isolamento, cultura e diferenciação de células do estroma da medula óssea e osteoclasto progenitores dos ratos
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Isolamento, cultura e diferenciação de células do estroma da medula óssea e osteoclasto progenitores dos ratos

Article DOI: 10.3791/56750-v • 08:07 min • January 6th, 2018
January 6th, 2018 •
1, 1, 1, 1
1Maine Medical Center Research Institute

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Neste artigo, apresentamos métodos para isolar e diferenciar as células do estroma da medula óssea e células-tronco hematopoiéticas de ossos longos de rato. Dois protocolos diferentes apresentam-se populações de células diferentes rendimento adequadas para a expansão e diferenciação em osteoblastos, osteoclastos e adipócitos.

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Biologia do desenvolvimento questão 131 Mesenchymal isolamento diferenciação osteoblastos adipócitos osteoclastos
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