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Retrograd Neuroanatomical sporing af Phrenic motoriske neuroner i mus
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Retrograd Neuroanatomical sporing af Phrenic motoriske neuroner i mus

Article DOI: 10.3791/56758-v • 09:05 min • February 22nd, 2018
February 22nd, 2018 •
1, 1, 1, 2, 2
1URVI, NARILIS, Université de Namur, 2URPhyM, NARILIS, Université de Namur

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Her, beskriver vi en protokol til at identificere phrenic motoriske neuroner i mus efter intrapleural levering af fluorophore konjugeret kolera toksin subunit beta. To teknikker er sammenlignet for at injicere den pleural hulrum: transdiaphragmatic versus transthoracic tilgange.

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Neurovidenskab sag 132 Phrenic motoriske neuroner retrograd mærkning intrapleural kolera toksin subunit beta mus neuroanatomical tracer
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