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JoVE Journal
Neuroscience

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Retrograde neuroanatomische tracering van Phrenic motorische neuronen in muizen
 
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Retrograde neuroanatomische tracering van Phrenic motorische neuronen in muizen

Article DOI: 10.3791/56758
February 22nd, 2018

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Summary February 22nd, 2018

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Hier beschrijven we een protocol voor het identificeren van phrenic motorische neuronen in muizen nadat pleurale levering van fluorophore geconjugeerd cholera-toxine subeenheid bèta. Twee technieken worden vergeleken om te injecteren de pleurale Holte: transdiaphragmatic versus transthoracic benaderingen.

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