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Journal / Developmental Biology / Generation af standardiserede og reproducerbare…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Generation af standardiserede og reproducerbare forhjernen-type Cerebral Organoids fra menneskelige induceret pluripotente stamceller
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Journal (Developmental Biology)
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Generation af standardiserede og reproducerbare forhjernen-type Cerebral Organoids fra menneskelige induceret pluripotente stamceller

Article DOI: 10.3791/56768-v • 10:25 min • January 23rd, 2018
January 23rd, 2018 •
*1, *1, 1, 1,2,3, 1
1Institute of Reconstructive Neurobiology, University of Bonn, 2Central Institute of Mental Health, University of Heidelberg/Medical Faculty Mannheim, 3Hector Institute for Translational Brain Research (HITBR gGmbH)
* These authors contributed equally

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Cerebral organoids repræsenterer en nye modelsystem til at undersøge tidlig menneskelige hjerne udvikling i vitro. Denne artikel giver den detaljeret metode til effektivt generere homogene dorsale forhjernen-type organoids fra menneskelige inducerede pluripotente stamceller herunder kritiske karakterisering og validering trin.

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Udviklingsmæssige biologi sag 131 Induced pluripotente stamceller neurobiologi cerebral organoids menneskelig kortikale udvikling kortikal misdannelser sygdom modellering
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