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JoVE Journal
Biochemistry

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毛细管电聚焦法对蛋白质及其亚型的高灵敏度和定量检测
 
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毛细管电聚焦法对蛋白质及其亚型的高灵敏度和定量检测

Article DOI: 10.3791/56794-v 07:58 min September 19th, 2018
September 19th, 2018

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Summary

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毛细管等电聚焦是一种基于抗体的、超灵敏的高通量技术, 它能够从极小的生物样品中详细描述蛋白质及其亚型。下面介绍了一种自动和机器人的方法, 用于检测和量化特定蛋白质及其亚型。

Tags

生物化学 问题 139 毛细管等电聚焦 信号转导 ERK1/2 抗体 化学发光 蛋白质亚型 蛋白质磷酸化 定量 超灵敏
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