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Journal / Neuroscience / Kvantitativ mätning av γ-sekretas-medierad…
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JoVE Journal Neuroscience
Kvantitativ mätning av γ-sekretas-medierad Amyloid Precursor Protein och Notch klyvning i cellbaserade luciferas Reporter Assay plattformar
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Kvantitativ mätning av γ-sekretas-medierad Amyloid Precursor Protein och Notch klyvning i cellbaserade luciferas Reporter Assay plattformar

Article DOI: 10.3791/56795-v • 06:40 min • January 25th, 2018
January 25th, 2018 •
*1, *1, *1, 1, 1, 1, 1
1Laboratory of Molecular Neurobiology, Institute of Cellular and Organismic Biology, Academia Sinica
* These authors contributed equally

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Vi har framgångsrikt genererade två substrat-specifika γ-sekretas analyser. Både cellbaserade analyser som presenteras här är utformade för att kvantifiera γ-sekretas enzymatiska aktiviteter via produktionen av firefly luciferas reportrar.

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Neurovetenskap fråga 131 Amyloid prekursor protein Notch γ-sekretas Firefly luciferas ErbB2 Alzheimers sjukdom Amyloid-β
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