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JoVE Journal
Developmental Biology

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利用荧光原位杂交 (fish) 监测中期和中期 I. 型果蝇卵母细胞的臂部凝聚状态
 
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利用荧光原位杂交 (fish) 监测中期和中期 I. 型果蝇卵母细胞的臂部凝聚状态

Article DOI: 10.3791/56802-v 12:46 min December 6th, 2017
December 6th, 2017

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Summary

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本手稿提出了一个详细的方法生成X-染色体臂探针和执行荧光原位杂交 (鱼), 以检查中期和中期我被捕的姐妹染色单体的内聚力状态果蝇卵母细胞。本协议适用于确定不同基因型的减数分裂手臂的内聚力是否完好或中断。

Tags

发育生物学 问题 130,果蝇 减数分裂 卵母细胞 姐妹染色单体内聚力 手臂凝聚力 荧光原位杂交 (鱼)
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