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JoVE Journal Developmental Biology
Met behulp van fluorescentie In Situ hybridisatie (FISH) te controleren van de toestand van Arm cohesie in Prometaphase en metafase ik Drosophila eicellen
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Met behulp van fluorescentie In Situ hybridisatie (FISH) te controleren van de toestand van Arm cohesie in Prometaphase en metafase ik Drosophila eicellen

Article DOI: 10.3791/56802-v • 12:46 min • December 6th, 2017
December 6th, 2017 •
1, 1
1Department of Biological Sciences, Dartmouth College

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Dit manuscript presenteert een gedetailleerde methode voor het genereren van X-chromosoom arm sondes en presterende fluorescentie in situ hybridisatie (FISH) te onderzoeken van de Braziliaanse deelstaat zus chromatide cohesie in prometaphase en metafase ik gearresteerd Drosophila eicellen. Dit protocol is geschikt om te bepalen of cohesie Meiotische arm intact of verstoord in verschillende genotypen is.

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Ontwikkelingsbiologie kwestie 130 Drosophila meiose oöcyt zuster chromatide cohesie en samenhang van de arm fluorescentie in situ hybridisatie (FISH)
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