Journal

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

DOI:

10.3791/56825-v

•

08:55 min

•

March 17, 2018

•

Floriana Cappiello, Bruno Casciaro, Maria Luisa Mangoni

¹Department of Biochemical Sciences,Sapienza University of Rome

Chapters

00:00Title
01:28Cell Preparation
03:34Cell Seeding in the Culture Inserts
04:30Pseudo-wound Healing Assay
05:52Image Analysis
06:53Representative Results
07:34Effects of Inhibitors on Cells Migration
08:33Conclusions

Summary

Automatic Translation

Here, we present a protocol to evaluate the effect of peptides on the migration of bronchial epithelial cells. This method allows for the rapid and highly reproducible obtainment of quantitative data on the speed of cell migration and wound closure.

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