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基于3D 荧光显微镜的病毒转录工厂功能成像

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy

JoVE Journal
Biology

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JoVE Journal Biology

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy

基于3D 荧光显微镜的病毒转录工厂功能成像

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6,996 Views

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09:03 min

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January 18, 2018

DOI:

10.3791/56832-v

DOI:

10.3791/56832-v

•

09:03 min

January 18, 2018

•

1 Views

Christopher P. Chen¹, Frank Chuang², Yoshihiro Izumiya¹

¹Department of Dermatology, University of California Davis School of Medicine,University of California, Davis, ²Center for Biophotonics, Department of Biochemistry and Molecular Medicine,University of California, Davis

Transcript

Summary

病毒转录工厂是分散的结构, 富含细胞 RNA 聚合酶 II, 以增加病毒基因转录在重新激活。本文介绍了一种通过免疫荧光染色和原位RNA 杂交技术在3D 核空间中主动转录病毒染色质位置的方法。

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