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JoVE Journal Genetics
CRISPR/Cas9-mediert målrettet integrering i Vivo med en homologi-mediert slutten begynte-basert strategi
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CRISPR/Cas9-mediert målrettet integrering i Vivo med en homologi-mediert slutten begynte-basert strategi

Article DOI: 10.3791/56844-v • 08:22 min • March 12th, 2018
March 12th, 2018 •
1,2, 1,2, 2,3,4, 1, 3, 1
1Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 2College of Life Sciences, University of Chinese Academy of Sciences, 3School of Life Science and Technology, Shanghai Tech University, 4Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

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De klynget regelmessig interspaced kort palindromic gjentar/CRISPR tilknyttet protein 9 (CRISPR/Cas9) gir et lovende verktøy for genteknologi, og åpner opp muligheten for målrettet integrasjon av effekter av transgener. Vi beskriver en homologi-mediert slutt begynte (HMEJ)-basert strategi for effektiv DNA målrettet integrering i vivo og målrettet gen-terapi bruker CRISPR/Cas9.

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Genetikk problemet 133 CRISPR/Cas9 målrettet integrasjon homologi-mediert slutten i Vivo Embryo genmodifiserte mus etter injeksjon
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