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Journal / Genetics / CRISPR/Cas9-vermittelten gezielte Integration In…
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JoVE Journal Genetics
CRISPR/Cas9-vermittelten gezielte Integration In Vivo mit einer Homologie-vermittelte Ende verbinden-basierte Strategie
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CRISPR/Cas9-vermittelten gezielte Integration In Vivo mit einer Homologie-vermittelte Ende verbinden-basierte Strategie

Article DOI: 10.3791/56844-v • 08:22 min • March 12th, 2018
March 12th, 2018 •
1,2, 1,2, 2,3,4, 1, 3, 1
1Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 2College of Life Sciences, University of Chinese Academy of Sciences, 3School of Life Science and Technology, Shanghai Tech University, 4Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

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Die gruppierten regelmäßig dazwischen kurze palindromische Wiederholungen/CRISPR verbundenen Protein 9 (CRISPR/Cas9) System bietet ein viel versprechendes Instrument für Gentechnik und eröffnet die Möglichkeit, gezielte Integration der transgene. Wir beschreiben eine Homologie-vermittelte Ende verbinden (HMEJ)-basierte Strategie für effiziente DNA Integration in Vivo gezielte und gezielte Gentherapien verwenden CRISPR/Cas9.

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Genetik Ausgabe 133 CRISPR/Cas9 gezielte Integration Homologie-vermittelte Ende kam In Vivo Embryo veränderte genetisch Mäuse hydrodynamische Injektion
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