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CRISPR/Cas9-medieret målrettede Integration In Vivo ved hjælp af en homologi-medieret End at deltage-baseret strategi
 
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CRISPR/Cas9-medieret målrettede Integration In Vivo ved hjælp af en homologi-medieret End at deltage-baseret strategi

Article DOI: 10.3791/56844
March 12th, 2018

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Summary March 12th, 2018

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De grupperede regelmæssigt interspaced korte palindromiske gentager/CRISPR forbundet protein 9 (CRISPR/Cas9) system giver et lovende redskab for genteknologi, og åbner op for muligheden for målrettede integration af transgener. Vi beskriver en homologi-medieret ende sammenføjning (HMEJ)-baseret strategi for effektiv DNA målrettet integration i vivo og målrettet genterapier bruger CRISPR/Cas9.

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