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Journal / Genetics / CRISPR/Cas9-medieret målrettede Integration In…
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JoVE Journal Genetics
CRISPR/Cas9-medieret målrettede Integration In Vivo ved hjælp af en homologi-medieret End at deltage-baseret strategi
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CRISPR/Cas9-medieret målrettede Integration In Vivo ved hjælp af en homologi-medieret End at deltage-baseret strategi

Article DOI: 10.3791/56844-v • 08:22 min • March 12th, 2018
March 12th, 2018 •
1,2, 1,2, 2,3,4, 1, 3, 1
1Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 2College of Life Sciences, University of Chinese Academy of Sciences, 3School of Life Science and Technology, Shanghai Tech University, 4Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

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De grupperede regelmæssigt interspaced korte palindromiske gentager/CRISPR forbundet protein 9 (CRISPR/Cas9) system giver et lovende redskab for genteknologi, og åbner op for muligheden for målrettede integration af transgener. Vi beskriver en homologi-medieret ende sammenføjning (HMEJ)-baseret strategi for effektiv DNA målrettet integration i vivo og målrettet genterapier bruger CRISPR/Cas9.

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Genetik sag 133 CRISPR/Cas9 målrettet Integration homologi-medieret ende tiltræder In Vivo Embryo genmodificerede mus hydrodynamiske injektion
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