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JoVE Journal
Biology

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16S rRNA-扩增子测序法对粪便微生物特性的指导协议
 
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16S rRNA-扩增子测序法对粪便微生物特性的指导协议

Article DOI: 10.3791/56845-v 08:05 min March 19th, 2018
March 19th, 2018

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Summary

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Click here for the English version.

这篇手稿描述了一个详细的标准化协议高通量 16S rRNA 扩增子测序。该协议引入了一个集成的, 统一的, 可行的, 廉价的协议, 从粪便样本收集, 通过数据分析。该协议允许分析大量的样本, 严格的标准和几个控制。

Tags

生物学 问题 133 微生物群 16S rRNA 测序 下一代测序 V4 变量区域 粪便样本 图书馆准备
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