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Guidet protokoll for Fecal mikrobiell karakterisering av 16S rRNA-Amplicon sekvenser
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Guidet protokoll for Fecal mikrobiell karakterisering av 16S rRNA-Amplicon sekvenser

Article DOI: 10.3791/56845-v • 08:05 min • March 19th, 2018
March 19th, 2018 •
1, 1, 1, 1, 1, 1, 2, 1,3, 1,4
1Sheba Medical Center, 2Children's Hospital of Pittsburgh of UPMC, 3Ariel University, 4Cincinnati Children's Hospital Medical Center

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Dette manuskriptet beskriver detaljert standardisert protokollen høy gjennomstrømming 16S rRNA-amplicon sekvenser. Protokollen introduserer en integrert, uniformerte, mulig og rimelig-protokoll fra fecal eksemplar samlingen gjennom data analyser. Denne protokollen aktiverer analyse av store antall prøver med strenge standarder og flere kontroller.

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Biologi problemet 133 Microbiome 16S rRNA sekvensering neste generasjons sekvensering V4 variable regionen fecal prøver bibliotek forberedelse
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