Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Erfassung der Interaktion Kinetics eines…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Erfassung der Interaktion Kinetics eines Ionenkanals Proteins mit kleinen Molekülen durch die Bio-Schicht Interferometrie Assay
Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells
Journal (Biochemistry)
Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells
Image Analysis to Identify Protein Crystals (Video) | JoVE
Journal (Biochemistry)
Image Analysis to Identify Protein Crystals (Video) | JoVE
 
Click here for the English version

Erfassung der Interaktion Kinetics eines Ionenkanals Proteins mit kleinen Molekülen durch die Bio-Schicht Interferometrie Assay

Article DOI: 10.3791/56846-v • 10:41 min • March 7th, 2018
March 7th, 2018 •
1, 2, 1, 3
1Department of General Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 2Key Laboratory of Systems Biomedicine (Ministry of Education), Institute of Systems Biomedicine, Shanghai Jiao Tong University, 3Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Hier das Protokoll beschreibt die Wechselwirkungen von gereinigten hEAG1 Ionen-Kanal-Protein mit niedermolekularer Lipid Liganden Phosphatidylinositol 4, 5-Bisphosphate (PIP-2). Die Messung zeigt, dass BLI eine mögliche Methode für neue kleine Molekül-Ion Channel Liganden Screening werden könnte.

Tags

Biochemie Ausgabe 133 Bio-Schicht Interferometrie (BLI) Ionen-Kanal-Protein kleines Molekül Affinität Proteinreinigung Säugetier-stabile Expressionssystems menschliche EAG1 (hEAG1) Kanal Arzneimittelforschung therapeutische Ziele Liganden Screening
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter