Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Um Western que mancha o protocolo para o pequeno…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Um Western que mancha o protocolo para o pequeno número de células-tronco hematopoiéticas
Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins
Journal (Biochemistry)
Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins
Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
Journal (Biochemistry)
Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
 
Click here for the English version

Um Western que mancha o protocolo para o pequeno número de células-tronco hematopoiéticas

Article DOI: 10.3791/56855-v • 08:45 min • August 22nd, 2018
August 22nd, 2018 •
1,2,3, 1, 2
1Division of Experimental Hematology, Cincinnati Children's Hospital Medical Center, 2Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, 3Department of Obstetrics and Gynecology, Southwest Hospital, Third Military Medical University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Um padrão ocidental que mancha o protocolo foi otimizado para analisar apenas 500 tronco hematopoiético ou células progenitoras. Otimização envolve cuidado, manipulação da amostra celular, limitando as transferências entre os tubos e lise diretamente as células no buffer de Laemmli sample.

Tags

Bioquímica edição 138 mancha ocidental células-tronco hematopoiéticas número de células pequenas SDS-PAGE reduzir a experimentação animal medula óssea
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter