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Journal / Bioengineering / Høj opløsning 3D Imaging af menneskelige…
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Bioengineering

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JoVE Journal Bioengineering
Høj opløsning 3D Imaging af menneskelige bugspytkirtlen Neuro-ø netværk
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Høj opløsning 3D Imaging af menneskelige bugspytkirtlen Neuro-ø netværk

Article DOI: 10.3791/56859-v • 09:54 min • January 29th, 2018
January 29th, 2018 •
1, 2, 3, 1, 1, 4, 5, 4, 1
1Department of Pathology, Immunology and Experimental Medicine, University of Florida, 2Heller School for Social Policy and Management, Brandeis University, 3Department of Medicine, College of Medicine, University of Florida, 4Department of Biomedical Engineering, College of Engineering, University of Florida, 5Department of Pediatrics, College of Medicine, University of Florida

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Vi præsenterer her, en protokol til billede menneskelige bugspytkirtlen sektioner i tre dimensioner (3D) ved hjælp af optimeret passiv clearing metoder. Dette manuskript viser disse procedurer for passiv optisk clearing efterfulgt af flere immunofluorescens farvning for at identificere nøgleelementerne i de autonome og sensoriske neurale netværk innerverer menneskelige Holme.

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Bioteknologi sag 131 bugspytkirtlen Holmen PAGTEN optisk clearing immunofluorescens insulin β-celler glukagon α-cellerne nerver Schwann celler GFAP Konfokal mikroskopi Lightsheet mikroskopi
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