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Bioengineering

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JoVE Journal Bioengineering
Hoge resolutie 3D beeldvorming van het Neuro-insulaire netwerk van menselijke alvleesklier
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Hoge resolutie 3D beeldvorming van het Neuro-insulaire netwerk van menselijke alvleesklier

Article DOI: 10.3791/56859-v • 09:54 min • January 29th, 2018
January 29th, 2018 •
1, 2, 3, 1, 1, 4, 5, 4, 1
1Department of Pathology, Immunology and Experimental Medicine, University of Florida, 2Heller School for Social Policy and Management, Brandeis University, 3Department of Medicine, College of Medicine, University of Florida, 4Department of Biomedical Engineering, College of Engineering, University of Florida, 5Department of Pediatrics, College of Medicine, University of Florida

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Hier presenteren we een protocol bij afbeelding menselijke alvleesklier secties in drie dimensies (3D) met behulp van geoptimaliseerde passieve methoden wissen. Dit manuscript toont deze procedures voor de passieve optische clearing gevolgd door meerdere immunofluorescentie kleuring ter identificatie van de belangrijkste elementen van de autonome en zintuiglijke neurale netwerken innervating menselijke eilandjes.

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Bioengineering kwestie 131 alvleesklier eilandje PACT optische clearing immunofluorescentie β-cellen insuline glucagon α-cellen zenuwen Schwann cellen GFAP confocal microscopie Lightsheet microscopie
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