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Journal / Bioengineering / Imagerie 3D à haute résolution du réseau…
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Bioengineering

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JoVE Journal Bioengineering
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Imagerie 3D à haute résolution du réseau Neuro-insulaire pancréas humain

Article DOI: 10.3791/56859-v • 09:54 min • January 29th, 2018
January 29th, 2018 •
1, 2, 3, 1, 1, 4, 5, 4, 1
1Department of Pathology, Immunology and Experimental Medicine, University of Florida, 2Heller School for Social Policy and Management, Brandeis University, 3Department of Medicine, College of Medicine, University of Florida, 4Department of Biomedical Engineering, College of Engineering, University of Florida, 5Department of Pediatrics, College of Medicine, University of Florida

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Nous présentons ici un passif de protocole aux sections pancréas humain image en trois dimensions (3D) à l’aide en optimisé méthodes de compensation. Ce manuscrit montre ces procédures de compensation optique passive, suivie par plusieurs immunofluorescence souillant pour identifier les éléments clés des réseaux neurones autonomes et sensitifs innervant les îlots humains.

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Bio-ingénierie numéro 131 pancréas îlots Pacte compensation optique immunofluorescence cellules β glucagon insuline α-cellules nerfs cellules de Schwann GFAP microscopie confocale microscopie de Lightsheet
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