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Journal / Bioengineering / Hochauflösende 3D Bildgebung des menschlichen…
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Bioengineering

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JoVE Journal Bioengineering
Hochauflösende 3D Bildgebung des menschlichen Bauchspeicheldrüse Neuro-insularen Netzwerks
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Hochauflösende 3D Bildgebung des menschlichen Bauchspeicheldrüse Neuro-insularen Netzwerks

Article DOI: 10.3791/56859-v • 09:54 min • January 29th, 2018
January 29th, 2018 •
1, 2, 3, 1, 1, 4, 5, 4, 1
1Department of Pathology, Immunology and Experimental Medicine, University of Florida, 2Heller School for Social Policy and Management, Brandeis University, 3Department of Medicine, College of Medicine, University of Florida, 4Department of Biomedical Engineering, College of Engineering, University of Florida, 5Department of Pediatrics, College of Medicine, University of Florida

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Hier präsentieren wir Ihnen einen Protokoll zum menschlichen Bauchspeicheldrüse Bildausschnitte in drei Dimensionen (3D) mit optimierten passiven clearing-Methoden. Diese Handschrift zeigt diese Verfahren für passive optische Clearing folgten mehrere Immunfluoreszenz Färbung um Schlüsselelemente der autonomen und sensorische neuronale Netze innervieren menschlichen Inselchen zu identifizieren.

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Frage 131 Bauchspeicheldrüse Bioengineering Inselchen Pakt optische clearing Immunfluoreszenz Insulin Glukagon β-Zellen α-Zellen Nerven Schwann-Zellen GFAP konfokale Mikroskopie Lichtscheibe Mikroskopie
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