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Journal / Bioengineering / Imagens 3D de alta resolução da rede…
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Bioengineering

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JoVE Journal Bioengineering
Imagens 3D de alta resolução da rede Neuro-insular pâncreas humano
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Imagens 3D de alta resolução da rede Neuro-insular pâncreas humano

Article DOI: 10.3791/56859-v • 09:54 min • January 29th, 2018
January 29th, 2018 •
1, 2, 3, 1, 1, 4, 5, 4, 1
1Department of Pathology, Immunology and Experimental Medicine, University of Florida, 2Heller School for Social Policy and Management, Brandeis University, 3Department of Medicine, College of Medicine, University of Florida, 4Department of Biomedical Engineering, College of Engineering, University of Florida, 5Department of Pediatrics, College of Medicine, University of Florida

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Aqui, apresentamos um passivo de protocolo para seções de pâncreas humano imagem em três dimensões (3D) usando otimizado métodos de compensação. Este manuscrito demonstra estes procedimentos para compensação de óptica passiva seguido por imunofluorescência múltiplas coloração para identificar os elementos-chave das redes neurais sensoriais e autonômicas, que inervam ilhotas humanas.

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Bioengenharia questão 131 pâncreas ilhéu pacto compensação óptica imunofluorescência insulina as células β o glucagon α-células nervos células de Schwann GFAP microscopia confocal microscopia Lightsheet
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