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Immunology and Infection

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JoVE Journal Immunology and Infection
Realizzazione di superfici di vetro di qualità ottica per studiare macrofago Fusion
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Realizzazione di superfici di vetro di qualità ottica per studiare macrofago Fusion

Article DOI: 10.3791/56866-v • 08:50 min • March 14th, 2018
March 14th, 2018 •
1,2, 3,4,5, 6, 7, 7, 8, 1,2, 3,4,5, 1,2
1Center for Metabolic and Vascular Biology, Mayo Clinic, 2Molecular and Cellular Biosciences, School of Life Sciences, Arizona State University, 3Department of Physics, Arizona State University, 4Center for Biological Physics, Arizona State University, 5Biodesign Institute, Arizona State University, 6Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 7Advanced Imaging Center, HHMI Janelia Research Campus, 8Advanced Light Microscopy Facility, European Molecular Biology Laboratory

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Questo protocollo descrive la realizzazione di superfici di vetro di qualità ottica adsorbito con composti contenenti idrocarburi a catena lunga che possono essere utilizzati per monitorare la fusione del macrofago di esemplari vivi e consente la microscopia di Super-risoluzione di campioni fissati .

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Ritrazione problema 133 formazione fusion multinucleated le cellule giganti del macrofago live imaging reazione da corpo estraneo cellule giganti di ente straniero infiammazione protocollo
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