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Journal / Developmental Biology / Protocolo de diferenciação cerebelar 3D…
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Developmental Biology

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JoVE Journal Developmental Biology
Protocolo de diferenciação cerebelar 3D simplificada com modificação 2D opcional
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Protocolo de diferenciação cerebelar 3D simplificada com modificação 2D opcional

Article DOI: 10.3791/56888-v • 10:15 min • December 9th, 2017
December 9th, 2017 •
1, 1,2
1Department of Pediatrics/Child Neurology, Amsterdam Neuroscience, VU University Medical Center, 2Department of Complex Trait Genetics, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam

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Descrevemos um meio de protocolo para hPSCs, usando definido de diferenciação 3D simplificado e reduzido os fatores de crescimento, capazes de gerar agregados de células com início neuroepithelial estruturas e positivos para marcadores cerebelar associada, bem como um opcional Modificação de 2D para diferenciar as células como um monolayer para gerar neurônios funcionais.

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Biologia do desenvolvimento edição 130 3D organoides células-tronco pluripotentes cerebelo célula grânulo córtex
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