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Journal / Developmental Biology / Strömlinjeformad 3D cerebellär differentiering…
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Developmental Biology

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JoVE Journal Developmental Biology
Strömlinjeformad 3D cerebellär differentiering protokoll med valfritt 2D modifiering
A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes
Journal (Developmental Biology)
A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes
Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility (Video) | JoVE
Journal (Developmental Biology)
Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility (Video) | JoVE
 
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Strömlinjeformad 3D cerebellär differentiering protokoll med valfritt 2D modifiering

Article DOI: 10.3791/56888-v • 10:15 min • December 9th, 2017
December 9th, 2017 •
1, 1,2
1Department of Pediatrics/Child Neurology, Amsterdam Neuroscience, VU University Medical Center, 2Department of Complex Trait Genetics, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam

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Vi beskriver ett förenklat 3D differentiering protokoll för hPSCs, med hjälp av definierade medium och minskade tillväxtfaktorer, kan generera cell aggregat med tidig neuroepithelial strukturer och positivt för cerebellär-associerade markörer, samt en valfri 2D modifiering för att skilja celler som en enskiktslager att generera funktionella nervceller.

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