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JoVE Journal
Developmental Biology

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rna 干扰和热休克诱导转录因子表达在重编程生殖细胞到神经元中的应用C. 线虫
 
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rna 干扰和热休克诱导转录因子表达在重编程生殖细胞到神经元中的应用C. 线虫

Article DOI: 10.3791/56889-v 07:54 min January 1st, 2018
January 1st, 2018

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Summary

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Click here for the English version.

本协议描述了如何在细胞命运转换期间研究在线虫线虫体内的细胞过程。利用转基因动物, 允许热休克启动子驱动的神经元的过度表达, 诱导转录因子 CHE-1 和 rna 介导的染色质调节因子 LIN-53 生殖细胞对神经元重新编程的损耗可以观察在体内

Tags

发育生物学 问题 131 生殖细胞 转录因子诱导重编程,线虫线虫 后生重编程屏障 RNA 干扰 热休克诱导 神经元重编程
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