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Journal / Biochemistry / Un metodo semplice per High Throughput Screening…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Un metodo semplice per High Throughput Screening chimico in Caenorhabditis Elegans
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Un metodo semplice per High Throughput Screening chimico in Caenorhabditis Elegans

Article DOI: 10.3791/56892-v • 08:49 min • March 20th, 2018
March 20th, 2018 •
1, 1, 2, 1
1The Buck Institute for Research on Aging, 2The Scripps Research Institute Center on Aging

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Qui descriviamo un protocollo semplice per produrre rapidamente centinaia di agar media di crescita del nematode, piastre di coltura 96 pozzetti con un numero consistente di Caenorhhabditis elegans per pozzetto. Queste culture sono utili per lo screening fenotipico di interi organismi. Qui ci concentriamo sull'utilizzo di queste culture ai prodotti chimici schermo per effetti di pro-longevità.

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Biochimica problema 133 durata della vita invecchiamento chimica biologia Screening Caenorhabditis alta velocità di trasmissione
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