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JoVE Journal Biochemistry
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
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Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Article DOI: 10.3791/56896-v • 11:22 min • January 30th, 2018
January 30th, 2018 •
1, 1, 1,2, 1
1Institute of Physical Chemistry, University of Freiburg, 2Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology

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Here, we present a protocol to obtain three-color smFRET data and its analysis with a 3D ensemble Hidden Markov Model. With this approach, scientists can extract kinetic information from complex protein systems, including cooperativity or correlated interactions.

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Three-color Single-molecule FRET Protein Interactions Dynamics Of Protein Systems Correlated Interactions Cooperativity Quantitative Biology Biologically Relevant Systems Flow Chamber Assembly Adhesive Film PEG Biotin PEG Functionalized Fused Quartz Slide Inlet And Outlet Holes Hot Plate Microscope Slide Cover Slip
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