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JoVE Journal Biochemistry
Uso del traste de una sola molécula a estudiar la correlación de las interacciones proteína
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Uso del traste de una sola molécula a estudiar la correlación de las interacciones proteína

Article DOI: 10.3791/56896-v • 11:22 min • January 30th, 2018
January 30th, 2018 •
1, 1, 1,2, 1
1Institute of Physical Chemistry, University of Freiburg, 2Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology

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Aquí, presentamos un protocolo para obtener datos smFRET tricolor y su análisis con un conjunto 3D modelo de Markov ocultos. Con este enfoque, los científicos pueden extraer información cinética de sistemas complejos de la proteína, incluyendo cooperatividad o interacción correlacionadas.

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Bioquímica número 131 biofísica cinética de la proteína multicolor smFRET TIRF HMM Hsp90 cooperatividad una sola molécula del traste
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