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Biochemistry

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JoVE Journal Biochemistry
Använda tre färger singel-molekyl bandet att studera korrelationen av proteininteraktioner
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
Journal (Biochemistry)
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
Journal (Biochemistry)
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
 
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Använda tre färger singel-molekyl bandet att studera korrelationen av proteininteraktioner

Article DOI: 10.3791/56896-v • 11:22 min • January 30th, 2018
January 30th, 2018 •
1, 1, 1,2, 1
1Institute of Physical Chemistry, University of Freiburg, 2Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology

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Här presenterar vi ett protokoll för att få tre färger smFRET data och dess analys med en 3D ensemble dold Markovmodell. Med detta synsätt, kan forskarna extrahera kinetiska information från komplexa protein system, inklusive kooperativitet eller korrelerade interaktioner.

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Fråga 131 protein kinetik biofysik biokemi flerfärgade smFRET Frida HMM Hsp90 kooperativitet singel-molekyl FRET
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